The aim of this research is to explore the differences and highlight the influencing factors in female and male scientists’ opinion and intention to create spin-off companies. In 2015, a survey in Hungary was conducted to gain a better insight into scientists’ intention to create spin-offs. Based on the theory of planned behaviour, which served as a theoretical and methodological framework, commercialization-related factors that were brought to light by gender and entrepreneurship studies were investigated. Most of the results are in line with the conclusions in international studies which find that female scientists express less willingness to create a spin-off company to commercialize research results. Furthermore, the results highlight that previous entrepreneurial experience, entrepreneurial competences, ties to industry and financial resources could also influence the intention to create a spin-off, suggesting that lack of these factors and competences hinders female scientists’ entrepreneurial endeavours.